diff --git a/pandas/core/computation/eval.py b/pandas/core/computation/eval.py
index a488aac08e060..b74f99fca21c7 100644
--- a/pandas/core/computation/eval.py
+++ b/pandas/core/computation/eval.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python3
 """
 Top level ``eval`` module.
 """
diff --git a/pandas/tests/io/generate_legacy_storage_files.py b/pandas/tests/io/generate_legacy_storage_files.py
index ca853ba5f00f5..e64103bd2cde8 100755
--- a/pandas/tests/io/generate_legacy_storage_files.py
+++ b/pandas/tests/io/generate_legacy_storage_files.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3
-
 """
 self-contained to write legacy storage pickle files
 
diff --git a/pandas/tests/plotting/common.py b/pandas/tests/plotting/common.py
index 75b825687209c..8e11ed83317df 100644
--- a/pandas/tests/plotting/common.py
+++ b/pandas/tests/plotting/common.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python3
 # coding: utf-8
 
 import os