diff --git a/.github/workflows/ubuntu.yml b/.github/workflows/ubuntu.yml
index 260c857e608b3..0e966f05bbc28 100644
--- a/.github/workflows/ubuntu.yml
+++ b/.github/workflows/ubuntu.yml
@@ -77,7 +77,6 @@ jobs:
           - name: "Numpy Dev"
             env_file: actions-310-numpydev.yaml
             pattern: "not slow and not network and not single_cpu"
-            pandas_testing_mode: "deprecate"
             test_args: "-W error::DeprecationWarning:numpy -W error::FutureWarning:numpy"
         exclude:
           - env_file: actions-39.yaml
@@ -96,7 +95,6 @@ jobs:
       EXTRA_APT: ${{ matrix.extra_apt || '' }}
       LANG: ${{ matrix.lang || '' }}
       LC_ALL: ${{ matrix.lc_all || '' }}
-      PANDAS_TESTING_MODE: ${{ matrix.pandas_testing_mode || '' }}
       PANDAS_DATA_MANAGER: ${{ matrix.pandas_data_manager || 'block' }}
       PANDAS_COPY_ON_WRITE: ${{ matrix.pandas_copy_on_write || '0' }}
       TEST_ARGS: ${{ matrix.test_args || '' }}
diff --git a/pandas/_testing/__init__.py b/pandas/_testing/__init__.py
index ce6b6ceea5604..9e95617e249c7 100644
--- a/pandas/_testing/__init__.py
+++ b/pandas/_testing/__init__.py
@@ -15,7 +15,6 @@
     Counter,
     Iterable,
 )
-import warnings
 
 import numpy as np
 
@@ -236,28 +235,6 @@
 
 EMPTY_STRING_PATTERN = re.compile("^$")
 
-# set testing_mode
-_testing_mode_warnings = (DeprecationWarning, ResourceWarning)
-
-
-def set_testing_mode() -> None:
-    # set the testing mode filters
-    testing_mode = os.environ.get("PANDAS_TESTING_MODE", "None")
-    if "deprecate" in testing_mode:
-        for category in _testing_mode_warnings:
-            warnings.simplefilter("always", category)
-
-
-def reset_testing_mode() -> None:
-    # reset the testing mode filters
-    testing_mode = os.environ.get("PANDAS_TESTING_MODE", "None")
-    if "deprecate" in testing_mode:
-        for category in _testing_mode_warnings:
-            warnings.simplefilter("ignore", category)
-
-
-set_testing_mode()
-
 
 def reset_display_options() -> None:
     """
@@ -1142,14 +1119,12 @@ def shares_memory(left, right) -> bool:
     "randbool",
     "rands",
     "reset_display_options",
-    "reset_testing_mode",
     "RNGContext",
     "round_trip_localpath",
     "round_trip_pathlib",
     "round_trip_pickle",
     "setitem",
     "set_locale",
-    "set_testing_mode",
     "set_timezone",
     "shares_memory",
     "SIGNED_INT_EA_DTYPES",
diff --git a/pandas/tests/io/pytables/conftest.py b/pandas/tests/io/pytables/conftest.py
index bbd815b9c6309..466e4ae8bb99c 100644
--- a/pandas/tests/io/pytables/conftest.py
+++ b/pandas/tests/io/pytables/conftest.py
@@ -2,18 +2,8 @@
 
 import pytest
 
-import pandas._testing as tm
-
 
 @pytest.fixture
 def setup_path():
     """Fixture for setup path"""
     return f"tmp.__{uuid.uuid4()}__.h5"
-
-
-@pytest.fixture(scope="module", autouse=True)
-def setup_mode():
-    """Reset testing mode fixture"""
-    tm.reset_testing_mode()
-    yield
-    tm.set_testing_mode()